Human GCNT1 ELISA Kit PicoKine®

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase is an enzyme that in humans is encoded by the GCNT1 gene. This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3 (GlcNAc beta 1-6) GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 105 103 112 107 4.61 4.3% Sample 2 204 222 241 236 226 11.51 5.1% Sample 3 389 342 415 444 398 22.26 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 105 103 112 107 4.61 4.3% Sample 1 109 105 103 112 107 4.61 4.3% Sample 2 204 222 241 236 226 11.51 5.1% Sample 2 204 222 241 236 226 11.51 5.1% Sample 3 389 342 415 444 398 22.26 5.6% Sample 3 389 342 415 444 398 22.26 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 204 389 116 214 472 Standard deviation 5.01 10.61 13.23 7.42 12.20 22.66 CV (%) 4.6% 5.2% 3.4% 6.4% 5.7% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 204 389 116 214 472 Standard deviation 5.01 10.61 13.23 7.42 12.20 22.66 CV (%) 4.6% 5.2% 3.4% 6.4% 5.7% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 109 204 389 116 214 472 Mean (pg/ml) 109 204 389 116 214 472 Standard deviation 5.01 10.61 13.23 7.42 12.20 22.66 Standard deviation 5.01 10.61 13.23 7.42 12.20 22.66 CV (%) 4.6% 5.2% 3.4% 6.4% 5.7% 4.8% CV (%) 4.6% 5.2% 3.4% 6.4% 5.7% 4.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.1038 0.167 0.292 0.519 0.865 1.48 2.312 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.1038 0.167 0.292 0.519 0.865 1.48 2.312 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.1038 0.167 0.292 0.519 0.865 1.48 2.312 O.D. 0.032 0.1038 0.167 0.292 0.519 0.865 1.48 2.312

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.