Human FOLR3 ELISA Kit PicoKine®

Folate receptor gamma is a protein that in humans is encoded by the FOLR3 gene. This gene encodes a member of the folate receptor (FOLR) family of proteins, which have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Expression of this gene may be elevated in ovarian and primary peritoneal carcinoma. This gene is present in a gene cluster on chromosome 11. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 43 48 52 54 49 2.86 5.8% Sample 2 115 121 108 98 111 4.97 4.5% Sample 3 180 186 199 203 192 11.71 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 43 48 52 54 49 2.86 5.8% Sample 1 43 48 52 54 49 2.86 5.8% Sample 2 115 121 108 98 111 4.97 4.5% Sample 2 115 121 108 98 111 4.97 4.5% Sample 3 180 186 199 203 192 11.71 6.1% Sample 3 180 186 199 203 192 11.71 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 43 115 180 52 107 205 Standard deviation 2.24 5.18 7.56 1.92 4.6 12.51 CV (%) 5.2% 4.5% 4.2% 3.7% 4.3% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 43 115 180 52 107 205 Standard deviation 2.24 5.18 7.56 1.92 4.6 12.51 CV (%) 5.2% 4.5% 4.2% 3.7% 4.3% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 43 115 180 52 107 205 Mean (pg/ml) 43 115 180 52 107 205 Standard deviation 2.24 5.18 7.56 1.92 4.6 12.51 Standard deviation 2.24 5.18 7.56 1.92 4.6 12.51 CV (%) 5.2% 4.5% 4.2% 3.7% 4.3% 6.1% CV (%) 5.2% 4.5% 4.2% 3.7% 4.3% 6.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.159 0.245 0.38 0.606 0.96 1.545 2.499 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.159 0.245 0.38 0.606 0.96 1.545 2.499 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.159 0.245 0.38 0.606 0.96 1.545 2.499 O.D. 0.051 0.159 0.245 0.38 0.606 0.96 1.545 2.499

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.