Human FGL1 ELISA Kit PicoKine®

Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FGL-1 is encoded by the FGL1 gene. Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 403 462 502 485 463 23.61 5.1% Sample 2 665 725 776 685 713 37.06 5.2% Sample 3 1490 1645 1550 1516 1550 74.41 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 403 462 502 485 463 23.61 5.1% Sample 1 403 462 502 485 463 23.61 5.1% Sample 2 665 725 776 685 713 37.06 5.2% Sample 2 665 725 776 685 713 37.06 5.2% Sample 3 1490 1645 1550 1516 1550 74.41 4.8% Sample 3 1490 1645 1550 1516 1550 74.41 4.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 403 665 1490 507 779 1865 Standard deviation 12.90 27.27 56.62 28.39 48.30 87.66 CV (%) 3.2% 4.1% 3.8% 5.6% 6.2% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 403 665 1490 507 779 1865 Standard deviation 12.90 27.27 56.62 28.39 48.30 87.66 CV (%) 3.2% 4.1% 3.8% 5.6% 6.2% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 403 665 1490 507 779 1865 Mean (pg/ml) 403 665 1490 507 779 1865 Standard deviation 12.90 27.27 56.62 28.39 48.30 87.66 Standard deviation 12.90 27.27 56.62 28.39 48.30 87.66 CV (%) 3.2% 4.1% 3.8% 5.6% 6.2% 4.7% CV (%) 3.2% 4.1% 3.8% 5.6% 6.2% 4.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.06 0.132 0.233 0.38 0.672 1.188 1.948 2.349 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.06 0.132 0.233 0.38 0.672 1.188 1.948 2.349 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.06 0.132 0.233 0.38 0.672 1.188 1.948 2.349 O.D. 0.06 0.132 0.233 0.38 0.672 1.188 1.948 2.349

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.