Human CD89 ELISA Kit PicoKine®

This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 25 29 27 26 1.69 6.5% Sample 2 67 68 75 88 75 3.13 4.2% Sample 3 267 296 270 311 286 13.16 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 25 29 27 26 1.69 6.5% Sample 1 23 25 29 27 26 1.69 6.5% Sample 2 67 68 75 88 75 3.13 4.2% Sample 2 67 68 75 88 75 3.13 4.2% Sample 3 267 296 270 311 286 13.16 4.6% Sample 3 267 296 270 311 286 13.16 4.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 67 267 25 70 275 Standard deviation 1.29 3.89 16.55 1.73 4.97 17.88 CV (%) 5.6% 5.8% 6.2% 6.9% 7.1% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 67 267 25 70 275 Standard deviation 1.29 3.89 16.55 1.73 4.97 17.88 CV (%) 5.6% 5.8% 6.2% 6.9% 7.1% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 23 67 267 25 70 275 Mean (pg/ml) 23 67 267 25 70 275 Standard deviation 1.29 3.89 16.55 1.73 4.97 17.88 Standard deviation 1.29 3.89 16.55 1.73 4.97 17.88 CV (%) 5.6% 5.8% 6.2% 6.9% 7.1% 6.5% CV (%) 5.6% 5.8% 6.2% 6.9% 7.1% 6.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.147 0.242 0.421 0.757 1.187 1.638 2.001 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.147 0.242 0.421 0.757 1.187 1.638 2.001 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.147 0.242 0.421 0.757 1.187 1.638 2.001 O.D. 0.017 0.147 0.242 0.421 0.757 1.187 1.638 2.001

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.