Human eNOS/NOS3 ELISA Kit PicoKine®

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme that in humans is encoded by the NOS3 gene located in the 7q35-7q36 region of chromosome 7. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 127 118 123 120 4.68 3.9% Sample 2 365 359 381 377 371 19.27 5.2% Sample 3 1362 1369 1489 1546 1442 31.71 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 127 118 123 120 4.68 3.9% Sample 1 112 127 118 123 120 4.68 3.9% Sample 2 365 359 381 377 371 19.27 5.2% Sample 2 365 359 381 377 371 19.27 5.2% Sample 3 1362 1369 1489 1546 1442 31.71 2.2% Sample 3 1362 1369 1489 1546 1442 31.71 2.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 365 1362 125 344 1399 Standard deviation 3.47 10.22 43.58 4.13 15.48 53.16 CV (%) 3.1% 2.8% 3.2% 3.3% 4.5% 3.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 365 1362 125 344 1399 Standard deviation 3.47 10.22 43.58 4.13 15.48 53.16 CV (%) 3.1% 2.8% 3.2% 3.3% 4.5% 3.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 365 1362 125 344 1399 Mean (pg/ml) 112 365 1362 125 344 1399 Standard deviation 3.47 10.22 43.58 4.13 15.48 53.16 Standard deviation 3.47 10.22 43.58 4.13 15.48 53.16 CV (%) 3.1% 2.8% 3.2% 3.3% 4.5% 3.8% CV (%) 3.1% 2.8% 3.2% 3.3% 4.5% 3.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.129 0.206 0.369 0.647 1.098 1.667 2.207 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.129 0.206 0.369 0.647 1.098 1.667 2.207 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.129 0.206 0.369 0.647 1.098 1.667 2.207 O.D. 0.037 0.129 0.206 0.369 0.647 1.098 1.667 2.207

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.