Human F11 ELISA Kit PicoKine®

Factor XI or plasma thromboplastin antecedent is the zymogen form of factor XIa, one of the enzymes of the coagulation cascade. Like many other coagulation factors, it is a serine protease. In humans, Factor XI is encoded by the F11 gene. This gene encodes coagulation factor XI of the blood coagulation cascade. This protein is present in plasma as a zymogen, which is a unique plasma coagulation enzyme because it exists as a homodimer consisting of two identical polypeptide chains linked by disulfide bonds. During activation of the plasma factor XI, an internal peptide bond is cleaved by factor XIIa (or XII) in each of the two chains, resulting in activated factor XIa, a serine protease composed of two heavy and two light chains held together by disulfide bonds. This activated plasma factor XI triggers the middle phase of the intrisic pathway of blood coagulation by activating factor IX. Defects in this factor lead to Rosenthal syndrome, a blood coagulation abnormality.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1652 1773 1679 1834 1735 76.32 4.4% Sample 2 3264 3645 3579 3421 3477 166.91 4.8% Sample 3 6973 6872 6721 7094 6915 359.58 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1652 1773 1679 1834 1735 76.32 4.4% Sample 1 1652 1773 1679 1834 1735 76.32 4.4% Sample 2 3264 3645 3579 3421 3477 166.91 4.8% Sample 2 3264 3645 3579 3421 3477 166.91 4.8% Sample 3 6973 6872 6721 7094 6915 359.58 5.2% Sample 3 6973 6872 6721 7094 6915 359.58 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1652 3264 6973 1687 3645 7524 Standard deviation 7269 159.94 362.6 92.79 167.67 428.87 CV (%) 4.4% 4.9% 5.2% 5.5% 4.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1652 3264 6973 1687 3645 7524 Standard deviation 7269 159.94 362.6 92.79 167.67 428.87 CV (%) 4.4% 4.9% 5.2% 5.5% 4.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1652 3264 6973 1687 3645 7524 Mean (pg/ml) 1652 3264 6973 1687 3645 7524 Standard deviation 7269 159.94 362.6 92.79 167.67 428.87 Standard deviation 7269 159.94 362.6 92.79 167.67 428.87 CV (%) 4.4% 4.9% 5.2% 5.5% 4.6% 5.7% CV (%) 4.4% 4.9% 5.2% 5.5% 4.6% 5.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.71 2.387 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.71 2.387 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.71 2.387 O.D. 0.027 0.113 0.179 0.297 0.533 0.979 1.71 2.387

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.