Human F10 ELISA Kit PicoKine®

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway) . The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1462 1658 1531 1596 1562 98.39 6.3% Sample 2 8562 8960 8216 8472 8553 496.05 5.8% Sample 3 20623 22361 21634 20478 21274 1255.17 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1462 1658 1531 1596 1562 98.39 6.3% Sample 1 1462 1658 1531 1596 1562 98.39 6.3% Sample 2 8562 8960 8216 8472 8553 496.05 5.8% Sample 2 8562 8960 8216 8472 8553 496.05 5.8% Sample 3 20623 22361 21634 20478 21274 1255.17 5.9% Sample 3 20623 22361 21634 20478 21274 1255.17 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1963 3865 21664 2014 413 21874 Standard deviation 106 224.17 866.56 126.88 26.02 1203.07 CV (%) 5.4% 5.8% 4% 6.3% 6.3% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1963 3865 21664 2014 413 21874 Standard deviation 106 224.17 866.56 126.88 26.02 1203.07 CV (%) 5.4% 5.8% 4% 6.3% 6.3% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1963 3865 21664 2014 413 21874 Mean (pg/ml) 1963 3865 21664 2014 413 21874 Standard deviation 106 224.17 866.56 126.88 26.02 1203.07 Standard deviation 106 224.17 866.56 126.88 26.02 1203.07 CV (%) 5.4% 5.8% 4% 6.3% 6.3% 5.5% CV (%) 5.4% 5.8% 4% 6.3% 6.3% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243 O.D. 0.067 0.179 0.254 0.444 0.704 1.245 1.739 2.243

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.