Human F7 ELISA Kit PicoKine®

Coagulation factor VII (EC 3.4.21.21, formerly known as proconvertin) is one of the proteins that causes blood to clot in the coagulation cascade, and in humans is coded for by the gene F7. This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1462 1658 1531 1596 1562 98.39 6.3% Sample 2 8562 8960 8216 8472 8553 496.05 5.8% Sample 3 20623 22361 21634 20478 21274 1255.17 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1462 1658 1531 1596 1562 98.39 6.3% Sample 1 1462 1658 1531 1596 1562 98.39 6.3% Sample 2 8562 8960 8216 8472 8553 496.05 5.8% Sample 2 8562 8960 8216 8472 8553 496.05 5.8% Sample 3 20623 22361 21634 20478 21274 1255.17 5.9% Sample 3 20623 22361 21634 20478 21274 1255.17 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1462 8562 20623 1503 8514 20114 Standard deviation 86.26 607.9 1319.87 84.17 434.21 1186.73 CV (%) 5.9% 7.1% 6.4% 5.6% 5.1% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1462 8562 20623 1503 8514 20114 Standard deviation 86.26 607.9 1319.87 84.17 434.21 1186.73 CV (%) 5.9% 7.1% 6.4% 5.6% 5.1% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1462 8562 20623 1503 8514 20114 Mean (pg/ml) 1462 8562 20623 1503 8514 20114 Standard deviation 86.26 607.9 1319.87 84.17 434.21 1186.73 Standard deviation 86.26 607.9 1319.87 84.17 434.21 1186.73 CV (%) 5.9% 7.1% 6.4% 5.6% 5.1% 5.9% CV (%) 5.9% 7.1% 6.4% 5.6% 5.1% 5.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.054 0.138 0.207 0.387 0.655 1.089 1.646 2.122 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.054 0.138 0.207 0.387 0.655 1.089 1.646 2.122 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.054 0.138 0.207 0.387 0.655 1.089 1.646 2.122 O.D. 0.054 0.138 0.207 0.387 0.655 1.089 1.646 2.122

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.