Human ERBB4 ELISA Kit PicoKine®

Receptor tyrosine-protein kinase erbB-4 is an enzyme that in humans is encoded by the ERBB4 gene. This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 36 48 59 44 1.81 4.1% Sample 2 149 190 155 162 164 5.9 3.6% Sample 3 868 841 796 822 832 39.09 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 36 48 59 44 1.81 4.1% Sample 1 34 36 48 59 44 1.81 4.1% Sample 2 149 190 155 162 164 5.9 3.6% Sample 2 149 190 155 162 164 5.9 3.6% Sample 3 868 841 796 822 832 39.09 4.7% Sample 3 868 841 796 822 832 39.09 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 149 868 46 182 951 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 149 868 46 182 951 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 149 868 46 182 951 Mean (pg/ml) 34 149 868 46 182 951 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 Standard deviation 1.5 7.3 37.32 2.58 9.28 52.31 CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5% CV (%) 4.4% 4.9% 4.3% 5.6% 5.1% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725 O.D. 0.044 0.131 0.206 0.333 0.55 0.974 1.649 2.725

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.