Human Calsyntenin 2 ELISA Kit PicoKine®

Predicted to enable calcium ion binding activity. Predicted to be involved in positive regulation of synapse assembly and positive regulation of synaptic transmission. Predicted to be located in postsynaptic density. Predicted to be active in cell surface; glutamatergic synapse; and postsynaptic membrane. Predicted to be integral component of postsynaptic density membrane.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 55 53 51 52 2.85 5.5% Sample 2 123 132 142 155 138 8.42 6.1% Sample 3 674 696 702 786 715 32.15 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 55 53 51 52 2.85 5.5% Sample 1 48 55 53 51 52 2.85 5.5% Sample 2 123 132 142 155 138 8.42 6.1% Sample 2 123 132 142 155 138 8.42 6.1% Sample 3 674 696 702 786 715 32.15 4.5% Sample 3 674 696 702 786 715 32.15 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 123 674 49 136 705 Standard deviation 3.36 8.49 43.81 2.89 7.89 45.12 CV (%) 7% 6.9% 6.5% 5.9% 5.8% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 123 674 49 136 705 Standard deviation 3.36 8.49 43.81 2.89 7.89 45.12 CV (%) 7% 6.9% 6.5% 5.9% 5.8% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 48 123 674 49 136 705 Mean (pg/ml) 48 123 674 49 136 705 Standard deviation 3.36 8.49 43.81 2.89 7.89 45.12 Standard deviation 3.36 8.49 43.81 2.89 7.89 45.12 CV (%) 7% 6.9% 6.5% 5.9% 5.8% 6.4% CV (%) 7% 6.9% 6.5% 5.9% 5.8% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.242 0.345 0.513 0.767 1.202 1.981 2.847 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.242 0.345 0.513 0.767 1.202 1.981 2.847 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.046 0.242 0.345 0.513 0.767 1.202 1.981 2.847 O.D. 0.046 0.242 0.345 0.513 0.767 1.202 1.981 2.847

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.