Human ENPP7 ELISA Kit PicoKine®

Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (E-NPP 7) also known as alkaline sphingomyelin phosphodiesterase (Alk-SMase) or intestinal alkaline sphingomyelinase is an enzyme that in humans is encoded by the ENPP7 gene. The protein encoded by this gene is an intestinal alkaline sphingomyelin phosphodiesterase that converts sphingomyelin to ceramide and phosphocholine. The encoded protein is anchored in the cell membrane, and it may function to protect the intestinal mucosa from inflammation and tumorigenesis. This protein is glycosylated and also exhibits lysophosphatidylcholine hydrolase activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 118 107 95 105 5.57 5.3% Sample 2 329 341 336 329 334 17.02 5.1% Sample 3 842 856 844 837 845 49 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 118 107 95 105 5.57 5.3% Sample 1 100 118 107 95 105 5.57 5.3% Sample 2 329 341 336 329 334 17.02 5.1% Sample 2 329 341 336 329 334 17.02 5.1% Sample 3 842 856 844 837 845 49 5.8% Sample 3 842 856 844 837 845 49 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 329 842 116 423 915 Standard deviation 5.5 17.44 51.36 5.22 20.73 51.24 CV (%) 5.5% 5.3% 6.1% 4.5% 4.9% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 329 842 116 423 915 Standard deviation 5.5 17.44 51.36 5.22 20.73 51.24 CV (%) 5.5% 5.3% 6.1% 4.5% 4.9% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 329 842 116 423 915 Mean (pg/ml) 100 329 842 116 423 915 Standard deviation 5.5 17.44 51.36 5.22 20.73 51.24 Standard deviation 5.5 17.44 51.36 5.22 20.73 51.24 CV (%) 5.5% 5.3% 6.1% 4.5% 4.9% 5.6% CV (%) 5.5% 5.3% 6.1% 4.5% 4.9% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163 O.D. 0.063 0.182 0.241 0.346 0.585 0.961 1.504 2.163

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.