Human DSG3 ELISA Kit PicoKine®

Desmoglein-3 is a protein that in humans is encoded by the DSG3 gene. This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 118 109 112 116 3.82 3.3% Sample 2 268 272 259 266 266 12.51 4.7% Sample 3 652 642 638 674 652 36.48 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 118 109 112 116 3.82 3.3% Sample 1 124 118 109 112 116 3.82 3.3% Sample 2 268 272 259 266 266 12.51 4.7% Sample 2 268 272 259 266 266 12.51 4.7% Sample 3 652 642 638 674 652 36.48 5.6% Sample 3 652 642 638 674 652 36.48 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 268 652 115 279 663 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 268 652 115 279 663 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 124 268 652 115 279 663 Mean (pg/ml) 124 268 652 115 279 663 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 Standard deviation 6.32 9.65 25.43 4.83 13.67 38.45 CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8% CV (%) 5.1% 3.6% 3.9% 4.2% 4.9% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451 O.D. 0.043 0.115 0.177 0.301 0.524 0.958 1.644 2.451

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.