Human DSG2 ELISA Kit PicoKine®

Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 782 752 741 736 753 38.39 5.1% Sample 2 1616 1721 1696 1745 1695 88.11 5.2% Sample 3 10536 11014 10145 10663 10590 518.89 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 782 752 741 736 753 38.39 5.1% Sample 1 782 752 741 736 753 38.39 5.1% Sample 2 1616 1721 1696 1745 1695 88.11 5.2% Sample 2 1616 1721 1696 1745 1695 88.11 5.2% Sample 3 10536 11014 10145 10663 10590 518.89 4.9% Sample 3 10536 11014 10145 10663 10590 518.89 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 782 1616 10536 754 1658 10641 Standard deviation 27.37 63.02 442.51 31.67 76.27 425.64 CV (%) 3.5% 3.9% 4.2% 4.2% 4.6% 4.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 782 1616 10536 754 1658 10641 Standard deviation 27.37 63.02 442.51 31.67 76.27 425.64 CV (%) 3.5% 3.9% 4.2% 4.2% 4.6% 4.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 782 1616 10536 754 1658 10641 Mean (pg/ml) 782 1616 10536 754 1658 10641 Standard deviation 27.37 63.02 442.51 31.67 76.27 425.64 Standard deviation 27.37 63.02 442.51 31.67 76.27 425.64 CV (%) 3.5% 3.9% 4.2% 4.2% 4.6% 4.0% CV (%) 3.5% 3.9% 4.2% 4.2% 4.6% 4.0%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.122 0.191 0.312 0.561 0.996 1.679 2.32 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.122 0.191 0.312 0.561 0.996 1.679 2.32 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.122 0.191 0.312 0.561 0.996 1.679 2.32 O.D. 0.035 0.122 0.191 0.312 0.561 0.996 1.679 2.32

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.