Human DSCAML1 ELISA Kit PicoKine®

Human DSCAML1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human DSCAML1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Down syndrome cell adhesion molecule like 1 is a protein in humans that is encoded by the DSCAML1 gene. The protein encoded by this gene is a member of the Ig superfamily of cell adhesion molecules and is involved in neuronal differentiation. The encoded membrane-bound protein localizes to the cell surface, where it forms aggregates that repel neuronal processes of the same cell type.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 268 196 228 231 231 12 5.2% Sample 2 1355 1403 1365 1396 1380 77.27 5.6% Sample 3 5652 5568 5463 5399 5521 264.98 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 268 196 228 231 231 12 5.2% Sample 1 268 196 228 231 231 12 5.2% Sample 2 1355 1403 1365 1396 1380 77.27 5.6% Sample 2 1355 1403 1365 1396 1380 77.27 5.6% Sample 3 5652 5568 5463 5399 5521 264.98 4.8% Sample 3 5652 5568 5463 5399 5521 264.98 4.8%

Gene Name

DSCAML1

Gene ID

57453

UniProt

Q8TD84

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E79-K1651

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 1355 5652 271 1403 5594 Standard deviation 6.16 48.78 180.86 11.11 58.93 246.14 CV (%) 2.3% 3.6% 3.2% 4.1% 4.2 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 268 1355 5652 271 1403 5594 Standard deviation 6.16 48.78 180.86 11.11 58.93 246.14 CV (%) 2.3% 3.6% 3.2% 4.1% 4.2 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 268 1355 5652 271 1403 5594 Mean (pg/ml) 268 1355 5652 271 1403 5594 Standard deviation 6.16 48.78 180.86 11.11 58.93 246.14 Standard deviation 6.16 48.78 180.86 11.11 58.93 246.14 CV (%) 2.3% 3.6% 3.2% 4.1% 4.2 4.4% CV (%) 2.3% 3.6% 3.2% 4.1% 4.2 4.4%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human DSCAML1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.095 0.19 0.415 0.799 1.335 1.569 2.013 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.095 0.19 0.415 0.799 1.335 1.569 2.013 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.095 0.19 0.415 0.799 1.335 1.569 2.013 O.D. 0.032 0.095 0.19 0.415 0.799 1.335 1.569 2.013

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.