Human DSC2 ELISA Kit PicoKine®

This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 198 216 223 218 11.13 5.1% Sample 2 1364 1352 1456 1316 1372 52.14 3.8% Sample 3 5315 5126 5241 5611 5323 218.25 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 198 216 223 218 11.13 5.1% Sample 1 236 198 216 223 218 11.13 5.1% Sample 2 1364 1352 1456 1316 1372 52.14 3.8% Sample 2 1364 1352 1456 1316 1372 52.14 3.8% Sample 3 5315 5126 5241 5611 5323 218.25 4.1% Sample 3 5315 5126 5241 5611 5323 218.25 4.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1364 5315 241 1354 5413 Standard deviation 17.46 94.12 340.16 14.94 79.89 373.5 CV (%) 7.4% 6.9% 6.4% 6.2% 5.9% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1364 5315 241 1354 5413 Standard deviation 17.46 94.12 340.16 14.94 79.89 373.5 CV (%) 7.4% 6.9% 6.4% 6.2% 5.9% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 236 1364 5315 241 1354 5413 Mean (pg/ml) 236 1364 5315 241 1354 5413 Standard deviation 17.46 94.12 340.16 14.94 79.89 373.5 Standard deviation 17.46 94.12 340.16 14.94 79.89 373.5 CV (%) 7.4% 6.9% 6.4% 6.2% 5.9% 6.9% CV (%) 7.4% 6.9% 6.4% 6.2% 5.9% 6.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.197 0.28 0.472 0.785 1.195 1.827 2.404 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.197 0.28 0.472 0.785 1.195 1.827 2.404 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.197 0.28 0.472 0.785 1.195 1.827 2.404 O.D. 0.057 0.197 0.28 0.472 0.785 1.195 1.827 2.404

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.