Human DDAH1 ELISA Kit PicoKine®

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 48 42 40 44 2.02 4.6% Sample 2 239 236 255 241 243 12.38 5.1% Sample 3 452 512 500 495 490 25.96 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 48 42 40 44 2.02 4.6% Sample 1 46 48 42 40 44 2.02 4.6% Sample 2 239 236 255 241 243 12.38 5.1% Sample 2 239 236 255 241 243 12.38 5.1% Sample 3 452 512 500 495 490 25.96 5.3% Sample 3 452 512 500 495 490 25.96 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 239 452 49 241 455 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 239 452 49 241 455 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 239 452 49 241 455 Mean (pg/ml) 46 239 452 49 241 455 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 Standard deviation 2.58 12.19 19.44 1.91 14.94 29.58 CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5% CV (%) 5.6% 5.1% 4.3% 3.9% 6.2% 6.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415 O.D. 0.02 0.109 0.181 0.33 0.593 1.06 1.864 2.415

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.