Mouse/Rat DCC ELISA Kit PicoKine®

Netrin receptor DCC, also known as DCC, or colorectal cancer suppressor is a protein which in humans is encoded by the DCC gene. Predicted to enable identical protein binding activity; netrin receptor activity; and transcription coactivator activity. Involved in postsynaptic modulation of chemical synaptic transmission. Acts upstream of or within generation of neurons. Located in axon. Is integral component of postsynaptic density membrane. Is active in Schaffer collateral - CA1 synapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; lung; and retina. Human ortholog (s) of this gene implicated in colorectal cancer; congenital mirror movement disorder; esophageal cancer; and esophagus squamous cell carcinoma. Orthologous to human DCC (DCC netrin 1 receptor) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 13 15 18 22 17 1.17 6.9% Sample 2 98 102 113 97 103 6.66 6.5% Sample 3 265 278 296 282 280 18.5 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 13 15 18 22 17 1.17 6.9% Sample 1 13 15 18 22 17 1.17 6.9% Sample 2 98 102 113 97 103 6.66 6.5% Sample 2 98 102 113 97 103 6.66 6.5% Sample 3 265 278 296 282 280 18.5 6.6% Sample 3 265 278 296 282 280 18.5 6.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 13 98 265 19 103 282 Standard deviation 0.81 6.17 15.64 0.91 5.25 15.51 CV (%) 6.2% 6.3% 5.9% 4.8% 5.1% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 13 98 265 19 103 282 Standard deviation 0.81 6.17 15.64 0.91 5.25 15.51 CV (%) 6.2% 6.3% 5.9% 4.8% 5.1% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 13 98 265 19 103 282 Mean (pg/ml) 13 98 265 19 103 282 Standard deviation 0.81 6.17 15.64 0.91 5.25 15.51 Standard deviation 0.81 6.17 15.64 0.91 5.25 15.51 CV (%) 6.2% 6.3% 5.9% 4.8% 5.1% 5.5% CV (%) 6.2% 6.3% 5.9% 4.8% 5.1% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.87 2.285 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.87 2.285 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.87 2.285 O.D. 0.021 0.157 0.271 0.483 0.814 1.311 1.87 2.285

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.