Human DCBLD2 ELISA Kit PicoKine®

Discoidin, CUB and LCCL domain-containing protein 2 is a protein that in humans is encoded by the DCBLD2 gene. Involved in negative regulation of cell growth and wound healing. Located in cell surface. Is integral component of plasma membrane.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 109 98 123 111 5 4.5% Sample 2 435 441 452 462 448 22.82 5.1% Sample 3 1216 1251 1263 1196 1232 68.96 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 109 98 123 111 5 4.5% Sample 1 114 109 98 123 111 5 4.5% Sample 2 435 441 452 462 448 22.82 5.1% Sample 2 435 441 452 462 448 22.82 5.1% Sample 3 1216 1251 1263 1196 1232 68.96 5.6% Sample 3 1216 1251 1263 1196 1232 68.96 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.