Human DCBLD2 ELISA Kit PicoKine®

Human DCBLD2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human DCBLD2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Discoidin, CUB and LCCL domain-containing protein 2 is a protein that in humans is encoded by the DCBLD2 gene. Involved in negative regulation of cell growth and wound healing. Located in cell surface. Is integral component of plasma membrane.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 109 98 123 111 5 4.5% Sample 2 435 441 452 462 448 22.82 5.1% Sample 3 1216 1251 1263 1196 1232 68.96 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 109 98 123 111 5 4.5% Sample 1 114 109 98 123 111 5 4.5% Sample 2 435 441 452 462 448 22.82 5.1% Sample 2 435 441 452 462 448 22.82 5.1% Sample 3 1216 1251 1263 1196 1232 68.96 5.6% Sample 3 1216 1251 1263 1196 1232 68.96 5.6%

Gene Name

DCBLD2

Gene ID

131566

UniProt

Q96PD2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q67-A528

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 435 1216 120 441 1268 Mean (pg/ml) 114 435 1216 120 441 1268 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 Standard deviation 5.02 18.71 47.42 7.68 28.67 73.54 CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8% CV (%) 4.4% 4.3% 3.9% 6.4% 6.5% 5.8%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human DCBLD2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558 O.D. 0.054 0.114 0.172 0.284 0.457 0.794 1.492 2.558

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.