Human CRELD1 ELISA Kit PicoKine®

Cysteine-rich with EGF-like domain protein 1 is a protein that in humans is encoded by the CRELD1 gene.This gene encodes a member of a subfamily of epidermal growth factor-related proteins. The encoded protein is characterized by a cysteine-rich with epidermal growth factor-like domain. This protein may function as a cell adhesion molecule. Mutations in this gene are the cause of atrioventricular septal defect. Alternate splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 95 88 97 93 4.17 4.5% Sample 2 368 375 363 384 373 18.25 4.9% Sample 3 952 988 971 967 970 56.23 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 95 88 97 93 4.17 4.5% Sample 1 91 95 88 97 93 4.17 4.5% Sample 2 368 375 363 384 373 18.25 4.9% Sample 2 368 375 363 384 373 18.25 4.9% Sample 3 952 988 971 967 970 56.23 5.8% Sample 3 952 988 971 967 970 56.23 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 368 952 92 349 925 Standard deviation 4.64 12.88 37.13 3.77 15.01 43.48 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 368 952 92 349 925 Standard deviation 4.64 12.88 37.13 3.77 15.01 43.48 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 91 368 952 92 349 925 Mean (pg/ml) 91 368 952 92 349 925 Standard deviation 4.64 12.88 37.13 3.77 15.01 43.48 Standard deviation 4.64 12.88 37.13 3.77 15.01 43.48 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.034 0.099 0.151 0.262 0.453 0.783 1.331 2.026 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.034 0.099 0.151 0.262 0.453 0.783 1.331 2.026 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.034 0.099 0.151 0.262 0.453 0.783 1.331 2.026 O.D. 0.034 0.099 0.151 0.262 0.453 0.783 1.331 2.026

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.