Human CPM ELISA Kit PicoKine®

Carboxypeptidase M (EC 3.4.17.12, CPM) is an enzyme.The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 112 108 103 107 2.78 2.6% Sample 2 468 453 450 466 459 16.07 3.5% Sample 3 1752 1821 1796 1804 1793 55.59 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 112 108 103 107 2.78 2.6% Sample 1 105 112 108 103 107 2.78 2.6% Sample 2 468 453 450 466 459 16.07 3.5% Sample 2 468 453 450 466 459 16.07 3.5% Sample 3 1752 1821 1796 1804 1793 55.59 3.1% Sample 3 1752 1821 1796 1804 1793 55.59 3.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 468 1752 123 470 1825 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 468 1752 123 470 1825 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 468 1752 123 470 1825 Mean (pg/ml) 105 468 1752 123 470 1825 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 Standard deviation 5.36 16.38 68.33 5.04 20.21 85.78 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927 O.D. 0.004 0.074 0.105 0.209 0.377 0.702 1.248 1.927

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.