Human COLEC12 ELISA Kit PicoKine®

Collectin-12, also known as collectin subfamily member 12, is a collectin protein that in humans is encoded by the COLEC12 gene.This gene encodes a member of the C-lectin family, proteins that possess collagen-like sequences and carbohydrate recognition domains. This protein is a scavenger receptor that displays several functions associated with host defense. It can bind to carbohydrate antigens on microorganisms, facilitating their recognition and removal. It also mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein by vascular endothelial cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 352 365 363 371 363 19.95 5.5% Sample 2 1277 1265 1302 1287 1283 75.68 5.9% Sample 3 4565 4542 4615 4787 4627 286.89 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 352 365 363 371 363 19.95 5.5% Sample 1 352 365 363 371 363 19.95 5.5% Sample 2 1277 1265 1302 1287 1283 75.68 5.9% Sample 2 1277 1265 1302 1287 1283 75.68 5.9% Sample 3 4565 4542 4615 4787 4627 286.89 6.2% Sample 3 4565 4542 4615 4787 4627 286.89 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 352 1277 4565 385 1365 4632 Standard deviation 19.36 71.51 146.08 23.49 94.19 213.07 CV (%) 5.5% 5.6% 3.2% 6.1% 6.9% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 352 1277 4565 385 1365 4632 Standard deviation 19.36 71.51 146.08 23.49 94.19 213.07 CV (%) 5.5% 5.6% 3.2% 6.1% 6.9% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 352 1277 4565 385 1365 4632 Mean (pg/ml) 352 1277 4565 385 1365 4632 Standard deviation 19.36 71.51 146.08 23.49 94.19 213.07 Standard deviation 19.36 71.51 146.08 23.49 94.19 213.07 CV (%) 5.5% 5.6% 3.2% 6.1% 6.9% 4.6% CV (%) 5.5% 5.6% 3.2% 6.1% 6.9% 4.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.066 0.117 0.234 0.424 0.775 1.388 2.165 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.066 0.117 0.234 0.424 0.775 1.388 2.165 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.066 0.117 0.234 0.424 0.775 1.388 2.165 O.D. 0.002 0.066 0.117 0.234 0.424 0.775 1.388 2.165

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.