Human Cathepsin Z ELISA Kit PicoKine®

Cathepsin Z, also called cathepsin X or cathepsin P, is a protein that in humans is encoded by the CTSZ gene. The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 468 471 455 462 464 28.3 6.1% Sample 2 1652 1672 1718 1695 1684 106.11 6.3% Sample 3 4527 4541 4612 4874 4639 255.12 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 468 471 455 462 464 28.3 6.1% Sample 1 468 471 455 462 464 28.3 6.1% Sample 2 1652 1672 1718 1695 1684 106.11 6.3% Sample 2 1652 1672 1718 1695 1684 106.11 6.3% Sample 3 4527 4541 4612 4874 4639 255.12 5.5% Sample 3 4527 4541 4612 4874 4639 255.12 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.3% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.3% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.3% 6% CV (%) 3.3% 3.5% 6.1% 4.5% 3.3% 6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.019 0.078 0.131 0.227 0.409 0.719 1.304 2.202 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.019 0.078 0.131 0.227 0.409 0.719 1.304 2.202 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.019 0.078 0.131 0.227 0.409 0.719 1.304 2.202 O.D. 0.019 0.078 0.131 0.227 0.409 0.719 1.304 2.202

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.