Human Cathepsin C ELISA Kit PicoKine®

Cathepsin C (CTSC) also known as dipeptidyl peptidase I (DPP-I) is a lysosomal exo-cysteine protease belonging to the peptidase C1 protein family, a subgroup of the cysteine cathepsins. In humans, it is encoded by the CTSC gene. This gene encodes a member of the peptidase C1 family and lysosomal cysteine proteinase that appears to be a central coordinator for activation of many serine proteinases in cells of the immune system. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate heavy and light chains that form a disulfide-linked dimer. A portion of the propeptide acts as an intramolecular chaperone for the folding and stabilization of the mature enzyme. This enzyme requires chloride ions for activity and can degrade glucagon. Defects in the encoded protein have been shown to be a cause of Papillon-Lefevre syndrome, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 170 199 227 189 5.19 2.8% Sample 2 430 397 487 407 430 25.43 5.9% Sample 3 4713 4044 3529 4523 4202 275.67 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 170 199 227 189 5.19 2.8% Sample 1 159 170 199 227 189 5.19 2.8% Sample 2 430 397 487 407 430 25.43 5.9% Sample 2 430 397 487 407 430 25.43 5.9% Sample 3 4713 4044 3529 4523 4202 275.67 6.6% Sample 3 4713 4044 3529 4523 4202 275.67 6.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 163 470 3588 258 505 3776 Standard deviation 4.56 32.15 267.66 10.06 12.47 219.01 CV (%) 2.8% 6.8% 7.5% 3.9% 2.5% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 163 470 3588 258 505 3776 Standard deviation 4.56 32.15 267.66 10.06 12.47 219.01 CV (%) 2.8% 6.8% 7.5% 3.9% 2.5% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 163 470 3588 258 505 3776 Mean (pg/ml) 163 470 3588 258 505 3776 Standard deviation 4.56 32.15 267.66 10.06 12.47 219.01 Standard deviation 4.56 32.15 267.66 10.06 12.47 219.01 CV (%) 2.8% 6.8% 7.5% 3.9% 2.5% 5.8% CV (%) 2.8% 6.8% 7.5% 3.9% 2.5% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.095 0.155 0.261 0.455 0.816 1.321 2.119 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.095 0.155 0.261 0.455 0.816 1.321 2.119 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.095 0.155 0.261 0.455 0.816 1.321 2.119 O.D. 0.036 0.095 0.155 0.261 0.455 0.816 1.321 2.119

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.