Human GM-CSFR ELISA Kit PicoKine®

The granulocyte-macrophage colony-stimulating factor receptor also known as CD116 (Cluster of Differentiation 116), is a receptor for granulocyte-macrophage colony-stimulating factor, which stimulates the production of white blood cells.The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 33 40 42 38 1.86 4.9% Sample 2 261 263 259 272 264 16.35 6.2% Sample 3 412 433 436 425 427 27.72 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 33 40 42 38 1.86 4.9% Sample 1 37 33 40 42 38 1.86 4.9% Sample 2 261 263 259 272 264 16.35 6.2% Sample 2 261 263 259 272 264 16.35 6.2% Sample 3 412 433 436 425 427 27.72 6.5% Sample 3 412 433 436 425 427 27.72 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 461 712 30 469 777 Standard deviation 1.92 26.28 34.18 1.95 33.3 46.62 CV (%) 6.2% 5.7% 4.8% 6.5% 7.1% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 461 712 30 469 777 Standard deviation 1.92 26.28 34.18 1.95 33.3 46.62 CV (%) 6.2% 5.7% 4.8% 6.5% 7.1% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 461 712 30 469 777 Mean (pg/ml) 31 461 712 30 469 777 Standard deviation 1.92 26.28 34.18 1.95 33.3 46.62 Standard deviation 1.92 26.28 34.18 1.95 33.3 46.62 CV (%) 6.2% 5.7% 4.8% 6.5% 7.1% 6% CV (%) 6.2% 5.7% 4.8% 6.5% 7.1% 6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.061 0.152 0.189 0.281 0.465 0.796 1.481 1.951 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.061 0.152 0.189 0.281 0.465 0.796 1.481 1.951 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.061 0.152 0.189 0.281 0.465 0.796 1.481 1.951 O.D. 0.061 0.152 0.189 0.281 0.465 0.796 1.481 1.951

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.