Human CD131 ELISA Kit PicoKine®

Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 41 45 39 1.29 3.3% Sample 2 181 190 177 188 184 8.65 4.7% Sample 3 469 465 447 472 463 25.94 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 41 45 39 1.29 3.3% Sample 1 32 38 41 45 39 1.29 3.3% Sample 2 181 190 177 188 184 8.65 4.7% Sample 2 181 190 177 188 184 8.65 4.7% Sample 3 469 465 447 472 463 25.94 5.6% Sample 3 469 465 447 472 463 25.94 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 181 469 34 199 483 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 181 469 34 199 483 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 181 469 34 199 483 Mean (pg/ml) 32 181 469 34 199 483 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4% CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.