Human CD131 ELISA Kit PicoKine®

Human CD131 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CSF2RB in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 41 45 39 1.29 3.3% Sample 2 181 190 177 188 184 8.65 4.7% Sample 3 469 465 447 472 463 25.94 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 41 45 39 1.29 3.3% Sample 1 32 38 41 45 39 1.29 3.3% Sample 2 181 190 177 188 184 8.65 4.7% Sample 2 181 190 177 188 184 8.65 4.7% Sample 3 469 465 447 472 463 25.94 5.6% Sample 3 469 465 447 472 463 25.94 5.6%

Gene Name

CSF2RB

Gene ID

1439

UniProt

P32927

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: W17-W443

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 181 469 34 199 483 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 181 469 34 199 483 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 181 469 34 199 483 Mean (pg/ml) 32 181 469 34 199 483 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 Standard deviation 1.5 13.76 29.08 2.45 14.13 35.74 CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4% CV (%) 4.7% 7.6% 6.2% 7.2% 7.1% 7.4%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant Human CSF2RB

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068 O.D. 0.017 0.163 0.256 0.399 0.624 0.995 1.506 2.068

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.