Human CNTFR ELISA Kit PicoKine®

The ciliary neurotrophic factor receptor, also known as CNTFR, binds the ciliary neurotrophic factor. This gene encodes a member of the type 1 cytokine receptor family. The encoded protein is the ligand-specific component of a tripartite receptor for ciliary neurotrophic factor, which plays a critical role in neuronal cell survival, differentiation and gene expression. Binding of ciliary neurotrophic factor to the encoded protein recruits the transmembrane components of the receptor, gp130 and leukemia inhibitory factor receptor, facilitating signal transduction. Single nucleotide polymorphisms in this gene may be associated with variations in muscle strength, as well as early onset of eating disorders. Alternatively spliced transcript variants have been observed for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 120 118 109 115 4.13 3.6% Sample 2 671 669 720 705 691 42.17 6.1% Sample 3 1458 1527 1469 1566 1505 66.22 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 120 118 109 115 4.13 3.6% Sample 1 112 120 118 109 115 4.13 3.6% Sample 2 671 669 720 705 691 42.17 6.1% Sample 2 671 669 720 705 691 42.17 6.1% Sample 3 1458 1527 1469 1566 1505 66.22 4.4% Sample 3 1458 1527 1469 1566 1505 66.22 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 671 1458 110 709 1522 Standard deviation 4.82 50.33 59.78 7.37 65.23 65.15 CV (%) 4.3% 7.5% 4.1% 6.7% 9.2% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 671 1458 110 709 1522 Standard deviation 4.82 50.33 59.78 7.37 65.23 65.15 CV (%) 4.3% 7.5% 4.1% 6.7% 9.2% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 671 1458 110 709 1522 Mean (pg/ml) 112 671 1458 110 709 1522 Standard deviation 4.82 50.33 59.78 7.37 65.23 65.15 Standard deviation 4.82 50.33 59.78 7.37 65.23 65.15 CV (%) 4.3% 7.5% 4.1% 6.7% 9.2% 4.3% CV (%) 4.3% 7.5% 4.1% 6.7% 9.2% 4.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.163 0.268 0.459 0.814 1.365 1.961 2.363 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.163 0.268 0.459 0.814 1.365 1.961 2.363 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.027 0.163 0.268 0.459 0.814 1.365 1.961 2.363 O.D. 0.027 0.163 0.268 0.459 0.814 1.365 1.961 2.363

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.