Human CASPR2 ELISA Kit PicoKine®

Contactin-associated protein-like 2 is a protein that in humans is encoded by the CNTNAP2 gene. This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons, and mediates interactions between neurons and glia during nervous system development and is also involved in localization of potassium channels within differentiating axons. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It is ly bound and regulated by forkhead box protein P2, a transcription factor related to speech and language development. This gene has been implicated in multiple neurodevelopmental disorders, including Gilles de la Tourette syndrome, schizophrenia, epilepsy, autism, ADHD and intellectual disability.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 15 14 11 13 0.42 3.2% Sample 2 45 48 52 57 51 1.41 2.8% Sample 3 189 192 208 196 196 7.65 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 12 15 14 11 13 0.42 3.2% Sample 1 12 15 14 11 13 0.42 3.2% Sample 2 45 48 52 57 51 1.41 2.8% Sample 2 45 48 52 57 51 1.41 2.8% Sample 3 189 192 208 196 196 7.65 3.9% Sample 3 189 192 208 196 196 7.65 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 45 189 13 47 206 Standard deviation 0.61 3.38 11.91 0.99 3.38 15.24 CV (%) 5.1% 7.5% 6.3% 7.6% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 12 45 189 13 47 206 Standard deviation 0.61 3.38 11.91 0.99 3.38 15.24 CV (%) 5.1% 7.5% 6.3% 7.6% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 12 45 189 13 47 206 Mean (pg/ml) 12 45 189 13 47 206 Standard deviation 0.61 3.38 11.91 0.99 3.38 15.24 Standard deviation 0.61 3.38 11.91 0.99 3.38 15.24 CV (%) 5.1% 7.5% 6.3% 7.6% 7.2% 7.4% CV (%) 5.1% 7.5% 6.3% 7.6% 7.2% 7.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.01 0.074 0.122 0.222 0.411 0.741 1.366 2.167 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.01 0.074 0.122 0.222 0.411 0.741 1.366 2.167 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.01 0.074 0.122 0.222 0.411 0.741 1.366 2.167 O.D. 0.01 0.074 0.122 0.222 0.411 0.741 1.366 2.167

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.