Mouse IL-18 ELISA Kit PicoKine®
Selected Variant
EK0433
Size: 96 Well With Removable Strips
Mouse IL-18 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il18 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Product Specifications
Background
Interleukin (IL) -18
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 54 57 60 56 1.44 2.6% Sample 2 286 302 292 297 294 9.12 3.1% Sample 3 905 880 869 892 887 24.42 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 54 57 60 56 1.44 2.6% Sample 1 51 54 57 60 56 1.44 2.6% Sample 2 286 302 292 297 294 9.12 3.1% Sample 2 286 302 292 297 294 9.12 3.1% Sample 3 905 880 869 892 887 24.42 2.8% Sample 3 905 880 869 892 887 24.42 2.8%
Gene Name
Il18
Gene ID
16173
UniProt
P70380
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N36-S192
Capture Antibody
A monoclonal antibody from rat
Detection Antibody
A biotinylated polyclonal antibody from goat
Applications
ELISA
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/mL - 2,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 286 905 59 310 933 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 286 905 59 310 933 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 51 286 905 59 310 933 Mean (pg/ml) 51 286 905 59 310 933 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 Standard deviation 2.14 11.15 41.63 3.07 17.05 45.72 CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9% CV (%) 4.2% 3.9% 4.6% 5.2% 5.5% 4.9%
Sensitivity
<10 pg/mL
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant Mouse Il18
Sample Data
Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138 O.D. 0.023 0.1 0.166 0.267 0.467 0.812 1.348 2.138
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
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