Human CFHR4 ELISA Kit PicoKine®

Complement factor H-related protein 4 is a protein that in humans is encoded by the CFHR4 gene. This gene is a member of the complement factor H (CFH) gene family, and encodes one of the 5 CFH-related (CFHR) proteins. These 5 genes are closely linked to the CFH gene on chromosome 1q31-q32. The CFHRs are secreted plasma proteins synthesized primarily by the hepatocytes, and composed of highly-related short consensus repeats (SCRs) . This protein enhances the cofactor activity of CFH, and is involved in complement regulation. It can associate with lipoproteins and may play a role in lipid metabolism. Alternatively spliced transcript variants encoding different isoforms (varying in the number of SCRs) have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 452 461 470 482 466 21.45 4.6% Sample 2 1663 1653 1743 1732 1698 54.33 3.2% Sample 3 4416 4612 4741 4674 4611 198.26 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 452 461 470 482 466 21.45 4.6% Sample 1 452 461 470 482 466 21.45 4.6% Sample 2 1663 1653 1743 1732 1698 54.33 3.2% Sample 2 1663 1653 1743 1732 1698 54.33 3.2% Sample 3 4416 4612 4741 4674 4611 198.26 4.3% Sample 3 4416 4612 4741 4674 4611 198.26 4.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 452 1663 4416 512 1698 4646 Standard deviation 23.05 58.21 172.22 20.99 73.01 218.36 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 452 1663 4416 512 1698 4646 Standard deviation 23.05 58.21 172.22 20.99 73.01 218.36 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 452 1663 4416 512 1698 4646 Mean (pg/ml) 452 1663 4416 512 1698 4646 Standard deviation 23.05 58.21 172.22 20.99 73.01 218.36 Standard deviation 23.05 58.21 172.22 20.99 73.01 218.36 CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7% CV (%) 5.1% 3.5% 3.9% 4.1% 4.3% 4.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.067 0.167 0.438 0.795 1.363 1.996 2.429 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.067 0.167 0.438 0.795 1.363 1.996 2.429 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.067 0.167 0.438 0.795 1.363 1.996 2.429 O.D. 0.035 0.067 0.167 0.438 0.795 1.363 1.996 2.429

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.