Human CFHR1 ELISA Kit PicoKine®

Complement factor H-related protein 1 is a protein that in humans is encoded by the CFHR1 gene. This gene encodes a secreted protein belonging to the complement factor H protein family. It binds to Pseudomonas aeruginosa elongation factor Tuf together with plasminogen, which is proteolytically activated. It is proposed that Tuf acts as a virulence factor by acquiring host proteins to the pathogen surface, controlling complement, and facilitating tissue invasion. Mutations in this gene are associated with an increased risk of atypical hemolytic-uremic syndrome.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 754 756 781 762 763 22.13 2.9% Sample 2 1625 1702 1698 1675 1675 43.55 2.6% Sample 3 10264 10126 10365 10612 10342 341.28 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 754 756 781 762 763 22.13 2.9% Sample 1 754 756 781 762 763 22.13 2.9% Sample 2 1625 1702 1698 1675 1675 43.55 2.6% Sample 2 1625 1702 1698 1675 1675 43.55 2.6% Sample 3 10264 10126 10365 10612 10342 341.28 3.3% Sample 3 10264 10126 10365 10612 10342 341.28 3.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 754 1625 10264 762 1645 10636 Standard deviation 52.03 94.25 667.16 48.01 88.83 659.43 CV (%) 6.9% 5.8% 6.5% 6.3% 5.4% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 754 1625 10264 762 1645 10636 Standard deviation 52.03 94.25 667.16 48.01 88.83 659.43 CV (%) 6.9% 5.8% 6.5% 6.3% 5.4% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 754 1625 10264 762 1645 10636 Mean (pg/ml) 754 1625 10264 762 1645 10636 Standard deviation 52.03 94.25 667.16 48.01 88.83 659.43 Standard deviation 52.03 94.25 667.16 48.01 88.83 659.43 CV (%) 6.9% 5.8% 6.5% 6.3% 5.4% 6.2% CV (%) 6.9% 5.8% 6.5% 6.3% 5.4% 6.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.115 0.186 0.345 0.614 1.131 1.733 2.439 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.115 0.186 0.345 0.614 1.131 1.733 2.439 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.035 0.115 0.186 0.345 0.614 1.131 1.733 2.439 O.D. 0.035 0.115 0.186 0.345 0.614 1.131 1.733 2.439

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.