Human CEACAM3 ELISA Kit PicoKine®

Carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) also known as CD66d (Cluster of Differentiation 66d), is a member of the carcinoembryonic antigen (CEA) gene family. This gene encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein s phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 26 27 29 26 1.33 5.1% Sample 2 164 154 177 167 166 8.11 4.9% Sample 3 436 485 452 446 455 21.37 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 26 27 29 26 1.33 5.1% Sample 1 22 26 27 29 26 1.33 5.1% Sample 2 164 154 177 167 166 8.11 4.9% Sample 2 164 154 177 167 166 8.11 4.9% Sample 3 436 485 452 446 455 21.37 4.7% Sample 3 436 485 452 446 455 21.37 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 164 436 27 172 424 Standard deviation 0.68 3.77 12.64 0.97 7.05 19.08 CV (%) 3.1% 2.3% 2.9% 3.6% 4.1% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 164 436 27 172 424 Standard deviation 0.68 3.77 12.64 0.97 7.05 19.08 CV (%) 3.1% 2.3% 2.9% 3.6% 4.1% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 22 164 436 27 172 424 Mean (pg/ml) 22 164 436 27 172 424 Standard deviation 0.68 3.77 12.64 0.97 7.05 19.08 Standard deviation 0.68 3.77 12.64 0.97 7.05 19.08 CV (%) 3.1% 2.3% 2.9% 3.6% 4.1% 4.5% CV (%) 3.1% 2.3% 2.9% 3.6% 4.1% 4.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937 O.D. 0.017 0.056 0.119 0.257 0.464 0.918 1.576 1.937

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.