Human DNAM-1/CD226 ELISA Kit PicoKine®

CD226 (Cluster of Differentiation 226), PTA1 (outdated term, 'platelet and T cell activation antigen 1') or DNAM-1 (DNAX Accessory Molecule-1) is a protein that in humans is encoded by the CD226 gene which is located on chromosome 18q22.3. This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 155 157 148 148 8.29 5.6% Sample 2 801 766 780 722 767 36.06 4.7% Sample 3 3566 3941 3852 3629 3747 172.36 4.65% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 155 157 148 148 8.29 5.6% Sample 1 132 155 157 148 148 8.29 5.6% Sample 2 801 766 780 722 767 36.06 4.7% Sample 2 801 766 780 722 767 36.06 4.7% Sample 3 3566 3941 3852 3629 3747 172.36 4.65% Sample 3 3566 3941 3852 3629 3747 172.36 4.65%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 801 3566 139 741 3657 Standard deviation 5.81 48.06 206.83 3.59 52.61 241.36 CV (%) 4.4% 6.0% 5.8% 6.9% 7.1% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 132 801 3566 139 741 3657 Standard deviation 5.81 48.06 206.83 3.59 52.61 241.36 CV (%) 4.4% 6.0% 5.8% 6.9% 7.1% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 132 801 3566 139 741 3657 Mean (pg/ml) 132 801 3566 139 741 3657 Standard deviation 5.81 48.06 206.83 3.59 52.61 241.36 Standard deviation 5.81 48.06 206.83 3.59 52.61 241.36 CV (%) 4.4% 6.0% 5.8% 6.9% 7.1% 6.6% CV (%) 4.4% 6.0% 5.8% 6.9% 7.1% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.033 0.098 0.149 0.253 0.428 0.76 1.413 2.023 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.033 0.098 0.149 0.253 0.428 0.76 1.413 2.023 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.033 0.098 0.149 0.253 0.428 0.76 1.413 2.023 O.D. 0.033 0.098 0.149 0.253 0.428 0.76 1.413 2.023

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.