Human CDSN ELISA Kit PicoKine®

Corneodesmosin is a protein that in humans is encoded by the CDSN gene. This gene encodes a protein found in corneodesmosomes, which localize to human epidermis and other cornified squamous epithelia. The encoded protein undergoes a series of cleavages during corneocyte maturation. This gene is highly polymorphic in human populations, and variation has been associated with skin diseases such as psoriasis, hypotrichosis and peeling skin syndrome. The gene is located in the major histocompatibility complex (MHC) class I region on chromosome 6.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 62 52 48 51 1.84 3.6% Sample 2 155 167 185 172 170 9.347 5.5% Sample 3 697 702 754 784 734 35.98 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 42 62 52 48 51 1.84 3.6% Sample 1 42 62 52 48 51 1.84 3.6% Sample 2 155 167 185 172 170 9.347 5.5% Sample 2 155 167 185 172 170 9.347 5.5% Sample 3 697 702 754 784 734 35.98 4.9% Sample 3 697 702 754 784 734 35.98 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 155 697 45 157 722 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 42 155 697 45 157 722 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 42 155 697 45 157 722 Mean (pg/ml) 42 155 697 45 157 722 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 Standard deviation 2.31 9.61 33.46 2.75 8.48 25.99 CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6% CV (%) 5.5% 6.2% 4.8% 6.1% 5.4% 3.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.85 1.525 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.85 1.525 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.85 1.525 O.D. 0.019 0.051 0.081 0.135 0.236 0.416 0.85 1.525

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.