Human CD34 ELISA Kit PicoKine®

CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species. The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 752 769 782 810 778 23.35 3.0% Sample 2 2754 2655 2896 2746 2763 176.82 6.4% Sample 3 6023 5940 6120 6454 6134 276.04 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 752 769 782 810 778 23.35 3.0% Sample 1 752 769 782 810 778 23.35 3.0% Sample 2 2754 2655 2896 2746 2763 176.82 6.4% Sample 2 2754 2655 2896 2746 2763 176.82 6.4% Sample 3 6023 5940 6120 6454 6134 276.04 4.5% Sample 3 6023 5940 6120 6454 6134 276.04 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 752 2754 6023 840 2865 7355 Standard deviation 49.63 176.26 427.63 49.56 186.23 485.43 CV (%) 6.6% 6.4% 7.1% 5.9% 6.5% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 752 2754 6023 840 2865 7355 Standard deviation 49.63 176.26 427.63 49.56 186.23 485.43 CV (%) 6.6% 6.4% 7.1% 5.9% 6.5% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 752 2754 6023 840 2865 7355 Mean (pg/ml) 752 2754 6023 840 2865 7355 Standard deviation 49.63 176.26 427.63 49.56 186.23 485.43 Standard deviation 49.63 176.26 427.63 49.56 186.23 485.43 CV (%) 6.6% 6.4% 7.1% 5.9% 6.5% 6.6% CV (%) 6.6% 6.4% 7.1% 5.9% 6.5% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.119 0.2 0.33 0.579 1.062 1.624 2.272 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.119 0.2 0.33 0.579 1.062 1.624 2.272 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.119 0.2 0.33 0.579 1.062 1.624 2.272 O.D. 0.031 0.119 0.2 0.33 0.579 1.062 1.624 2.272

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.