Human sCD2 ELISA Kit PicoKine®

CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 157 155 149 149 9.67 6.5% Sample 2 815 800 742 766 781 36.7 4.7% Sample 3 3563 3565 3941 3594 3666 150.3 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 157 155 149 149 9.67 6.5% Sample 1 134 157 155 149 149 9.67 6.5% Sample 2 815 800 742 766 781 36.7 4.7% Sample 2 815 800 742 766 781 36.7 4.7% Sample 3 3563 3565 3941 3594 3666 150.3 4.1% Sample 3 3563 3565 3941 3594 3666 150.3 4.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 815 3563 141 789 3651 Standard deviation 8.44 43.2 167.46 10.01 48.92 200.81 CV (%) 6.3% 5.3% 4.7% 7.1% 6.2% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 815 3563 141 789 3651 Standard deviation 8.44 43.2 167.46 10.01 48.92 200.81 CV (%) 6.3% 5.3% 4.7% 7.1% 6.2% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 134 815 3563 141 789 3651 Mean (pg/ml) 134 815 3563 141 789 3651 Standard deviation 8.44 43.2 167.46 10.01 48.92 200.81 Standard deviation 8.44 43.2 167.46 10.01 48.92 200.81 CV (%) 6.3% 5.3% 4.7% 7.1% 6.2% 5.5% CV (%) 6.3% 5.3% 4.7% 7.1% 6.2% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.016 0.084 0.162 0.255 0.45 0.831 1.445 2.087 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.016 0.084 0.162 0.255 0.45 0.831 1.445 2.087 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.016 0.084 0.162 0.255 0.45 0.831 1.445 2.087 O.D. 0.016 0.084 0.162 0.255 0.45 0.831 1.445 2.087

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.