Human CD302 ELISA Kit PicoKine®

The CD302 antigen also known as C-type lectin domain family 13 member A is a protein that in humans is encoded by the CD302 gene. CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 136 142 133 129 135 6.62 4.9% Sample 2 252 258 261 274 261 9.41 3.6% Sample 3 548 569 582 603 576 18.42 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 136 142 133 129 135 6.62 4.9% Sample 1 136 142 133 129 135 6.62 4.9% Sample 2 252 258 261 274 261 9.41 3.6% Sample 2 252 258 261 274 261 9.41 3.6% Sample 3 548 569 582 603 576 18.42 3.2% Sample 3 548 569 582 603 576 18.42 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 136 252 548 147 274 559 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 136 252 548 147 274 559 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 136 252 548 147 274 559 Mean (pg/ml) 136 252 548 147 274 559 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 Standard deviation 5.98 13.1 18.08 7.2 16.71 30.19 CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4% CV (%) 4.4% 5.2% 3.3% 4.9% 6.1% 5.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854 O.D. 0.021 0.062 0.103 0.171 0.365 0.665 1.155 1.854

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.