Human CD300LG ELISA Kit PicoKine®

Human CD300LG ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD300LG in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Members of the CD300-like (CD300L) family, such as CD300LG, are widely expressed on hematopoietic cells. All CD300L proteins are type I cell surface glycoproteins that contain a single immunoglobulin (Ig) V-like domain.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 58 49 44 52 1.71 3.3% Sample 2 202 186 188 208 196 6.86 3.5% Sample 3 754 752 789 839 784 32.12 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 58 49 44 52 1.71 3.3% Sample 1 56 58 49 44 52 1.71 3.3% Sample 2 202 186 188 208 196 6.86 3.5% Sample 2 202 186 188 208 196 6.86 3.5% Sample 3 754 752 789 839 784 32.12 4.1% Sample 3 754 752 789 839 784 32.12 4.1%

Gene Name

CD300LG

Gene ID

146894

UniProt

Q6UXG3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L19-L249

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 202 754 60 159 747 Standard deviation 2.69 11.92 38.45 4.02 10.34 44.82 CV (%) 4.8% 5.9% 5.1% 6.7% 6.5% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 202 754 60 159 747 Standard deviation 2.69 11.92 38.45 4.02 10.34 44.82 CV (%) 4.8% 5.9% 5.1% 6.7% 6.5% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 202 754 60 159 747 Mean (pg/ml) 56 202 754 60 159 747 Standard deviation 2.69 11.92 38.45 4.02 10.34 44.82 Standard deviation 2.69 11.92 38.45 4.02 10.34 44.82 CV (%) 4.8% 5.9% 5.1% 6.7% 6.5% 6% CV (%) 4.8% 5.9% 5.1% 6.7% 6.5% 6%

Sensitivity

<20 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CD300LG

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.139 0.234 0.424 0.783 1.281 1.819 2.237 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.139 0.234 0.424 0.783 1.281 1.819 2.237 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.139 0.234 0.424 0.783 1.281 1.819 2.237 O.D. 0.031 0.139 0.234 0.424 0.783 1.281 1.819 2.237

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.