Human CD79B ELISA Kit PicoKine®

CD79b molecule, immunoglobulin-associated beta, also known as CD79B (Cluster of Differentiation 79B), is a human gene. The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig) . Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 39 41 40 41 2.3 5.6% Sample 2 235 253 256 227 243 10.2 4.2% Sample 3 453 528 500 506 497 25.83 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 39 41 40 41 2.3 5.6% Sample 1 44 39 41 40 41 2.3 5.6% Sample 2 235 253 256 227 243 10.2 4.2% Sample 2 235 253 256 227 243 10.2 4.2% Sample 3 453 528 500 506 497 25.83 5.2% Sample 3 453 528 500 506 497 25.83 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 235 453 47 240 445 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 235 453 47 240 445 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 235 453 47 240 445 Mean (pg/ml) 44 235 453 47 240 445 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 Standard deviation 2.9 18.1 25.37 2.77 14.88 30.26 CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8% CV (%) 6.6% 7.7% 5.6% 5.9% 6.2% 6.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107 O.D. 0.02 0.061 0.128 0.236 0.412 0.835 1.39 2.107

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.