Human CD58 ELISA Kit PicoKine®

CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 312 294 277 292 16.06 5.5% Sample 2 1532 1522 1556 1649 1565 87.63 5.6% Sample 3 5364 5841 5964 6011 5795 399.86 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 285 312 294 277 292 16.06 5.5% Sample 1 285 312 294 277 292 16.06 5.5% Sample 2 1532 1522 1556 1649 1565 87.63 5.6% Sample 2 1532 1522 1556 1649 1565 87.63 5.6% Sample 3 5364 5841 5964 6011 5795 399.86 6.9% Sample 3 5364 5841 5964 6011 5795 399.86 6.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1532 5634 296 1602 5648 Standard deviation 9.41 55.15 241.38 15.39 62.48 271.1 CV (%) 3.3% 3.6% 4.5% 5.2% 3.9% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 285 1532 5634 296 1602 5648 Standard deviation 9.41 55.15 241.38 15.39 62.48 271.1 CV (%) 3.3% 3.6% 4.5% 5.2% 3.9% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 285 1532 5634 296 1602 5648 Mean (pg/ml) 285 1532 5634 296 1602 5648 Standard deviation 9.41 55.15 241.38 15.39 62.48 271.1 Standard deviation 9.41 55.15 241.38 15.39 62.48 271.1 CV (%) 3.3% 3.6% 4.5% 5.2% 3.9% 4.8% CV (%) 3.3% 3.6% 4.5% 5.2% 3.9% 4.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.099 0.148 0.299 0.518 0.958 1.647 2.304 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.099 0.148 0.299 0.518 0.958 1.647 2.304 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.099 0.148 0.299 0.518 0.958 1.647 2.304 O.D. 0.008 0.099 0.148 0.299 0.518 0.958 1.647 2.304

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.