Human CD109 ELISA Kit PicoKine®

This gene encodes a glycosyl phosphatidylinositol (GPI) -linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta) . Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 468 470 489 505 483 21.74 4.5% Sample 2 1652 1744 1825 1697 1730 72.64 4.2% Sample 3 4527 4652 4741 4426 4587 288.95 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 468 470 489 505 483 21.74 4.5% Sample 1 468 470 489 505 483 21.74 4.5% Sample 2 1652 1744 1825 1697 1730 72.64 4.2% Sample 2 1652 1744 1825 1697 1730 72.64 4.2% Sample 3 4527 4652 4741 4426 4587 288.95 6.3% Sample 3 4527 4652 4741 4426 4587 288.95 6.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 468 1652 4527 521 1699 4651 Mean (pg/ml) 468 1652 4527 521 1699 4651 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 Standard deviation 15.44 57.82 276.15 23.45 54.37 279.06 CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6% CV (%) 3.3% 3.5% 6.1% 4.5% 3.2% 6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355 O.D. 0.049 0.144 0.221 0.371 0.656 1.143 1.803 2.355

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.