Human CA13 ELISA Kit PicoKine®

Human CA13 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CA13 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carbonic anhydrase 13 is a protein that in humans is encoded by the CA13 gene. Carbonic anhydrases (CAs) are a family of zinc metalloenzymes. For background information on the CA family, see MIM 114800.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 103 125 118 127 118 8.16 6.9% Sample 2 452 463 504 489 477 33.87 7.1% Sample 3 1655 1788 1692 1701 1709 88.87 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 103 125 118 127 118 8.16 6.9% Sample 1 103 125 118 127 118 8.16 6.9% Sample 2 452 463 504 489 477 33.87 7.1% Sample 2 452 463 504 489 477 33.87 7.1% Sample 3 1655 1788 1692 1701 1709 88.87 5.2% Sample 3 1655 1788 1692 1701 1709 88.87 5.2%

Gene Name

CA13

Gene ID

377677

UniProt

Q8N1Q1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-H262

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 103 452 1655 122 484 1592 Standard deviation 6.8 24.41 86.06 5.49 23.72 97.11 CV (%) 6.6% 5.4% 5.2% 4.5% 4.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 103 452 1655 122 484 1592 Standard deviation 6.8 24.41 86.06 5.49 23.72 97.11 CV (%) 6.6% 5.4% 5.2% 4.5% 4.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 103 452 1655 122 484 1592 Mean (pg/ml) 103 452 1655 122 484 1592 Standard deviation 6.8 24.41 86.06 5.49 23.72 97.11 Standard deviation 6.8 24.41 86.06 5.49 23.72 97.11 CV (%) 6.6% 5.4% 5.2% 4.5% 4.9% 6.1% CV (%) 6.6% 5.4% 5.2% 4.5% 4.9% 6.1%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CA13

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.027 0.136 0.24 0.439 0.741 1.222 1.861 2.441 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.027 0.136 0.24 0.439 0.741 1.222 1.861 2.441 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.027 0.136 0.24 0.439 0.741 1.222 1.861 2.441 O.D. 0.027 0.136 0.24 0.439 0.741 1.222 1.861 2.441

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.