Human complement C3a ELISA Kit PicoKine®

Complement component 3, often simply called C3, is a protein of the immune system. In humans it is encoded on chromosome 19 by a gene called C3. Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 63 61 62 65 63 3.83 6.1% Sample 2 213 195 232 215 214 11.97 5.6% Sample 3 1095 1045 1002 989 1033 58.87 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 63 61 62 65 63 3.83 6.1% Sample 1 63 61 62 65 63 3.83 6.1% Sample 2 213 195 232 215 214 11.97 5.6% Sample 2 213 195 232 215 214 11.97 5.6% Sample 3 1095 1045 1002 989 1033 58.87 5.7% Sample 3 1095 1045 1002 989 1033 58.87 5.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 63 213 1095 60 224 1088 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 63 213 1095 60 224 1088 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 63 213 1095 60 224 1088 Mean (pg/ml) 63 213 1095 60 224 1088 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 Standard deviation 3.28 14.06 50.37 3.06 14.34 38.08 CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5% CV (%) 5.2% 6.6% 4.6% 5.1% 6.4% 3.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004 O.D. 0.01 0.182 0.295 0.471 0.733 1.108 1.808 3.004

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.