Human Complement C2 ELISA Kit PicoKine®

Complement C2 is a protein that in humans is encoded by the C2 gene. Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 785 774 769 782 778 22.55 2.9% Sample 2 1654 1723 1645 1697 1680 43.67 2.6% Sample 3 10235 10156 10619 10665 10306 371.03 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 785 774 769 782 778 22.55 2.9% Sample 1 785 774 769 782 778 22.55 2.9% Sample 2 1654 1723 1645 1697 1680 43.67 2.6% Sample 2 1654 1723 1645 1697 1680 43.67 2.6% Sample 3 10235 10156 10619 10665 10306 371.03 3.6% Sample 3 10235 10156 10619 10665 10306 371.03 3.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 785 1654 10235 766 1641 10634 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 785 1654 10235 766 1641 10634 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 785 1654 10235 766 1641 10634 Mean (pg/ml) 785 1654 10235 766 1641 10634 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 Standard deviation 35.33 71.12 521.99 24.51 59.08 521.07 CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9% CV (%) 4.5% 4.3% 5.1% 3.2% 3.6% 4.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.