Mouse BID ELISA Kit PicoKine®

The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the Bcl-2 protein family. This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8) ; CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 49 61 55 53 3.05 5.8% Sample 2 265 285 290 296 284 11.08 3.9% Sample 3 1125 1325 1266 1451 1292 63.3 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 49 61 55 53 3.05 5.8% Sample 1 45 49 61 55 53 3.05 5.8% Sample 2 265 285 290 296 284 11.08 3.9% Sample 2 265 285 290 296 284 11.08 3.9% Sample 3 1125 1325 1266 1451 1292 63.3 4.9% Sample 3 1125 1325 1266 1451 1292 63.3 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 265 1125 55 266 1155 Standard deviation 2.03 14.58 67.5 3.36 13.03 64.68 CV (%) 4.5% 5.5% 6% 6.1% 4.9% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 265 1125 55 266 1155 Standard deviation 2.03 14.58 67.5 3.36 13.03 64.68 CV (%) 4.5% 5.5% 6% 6.1% 4.9% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 45 265 1125 55 266 1155 Mean (pg/ml) 45 265 1125 55 266 1155 Standard deviation 2.03 14.58 67.5 3.36 13.03 64.68 Standard deviation 2.03 14.58 67.5 3.36 13.03 64.68 CV (%) 4.5% 5.5% 6% 6.1% 4.9% 5.6% CV (%) 4.5% 5.5% 6% 6.1% 4.9% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.102 0.15 0.243 0.421 0.765 1.383 2.228 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.102 0.15 0.243 0.421 0.765 1.383 2.228 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.036 0.102 0.15 0.243 0.421 0.765 1.383 2.228 O.D. 0.036 0.102 0.15 0.243 0.421 0.765 1.383 2.228

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.