Human ACY1 ELISA Kit PicoKine®

Aminoacylase-1 is an enzyme that in humans is encoded by the ACY1 gene. This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 457 488 462 454 465 15.35 3.3% Sample 2 2654 2741 2679 2546 2655 119.48 4.5% Sample 3 8622 8845 8961 8753 8795 448.56 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 457 488 462 454 465 15.35 3.3% Sample 1 457 488 462 454 465 15.35 3.3% Sample 2 2654 2741 2679 2546 2655 119.48 4.5% Sample 2 2654 2741 2679 2546 2655 119.48 4.5% Sample 3 8622 8845 8961 8753 8795 448.56 5.1% Sample 3 8622 8845 8961 8753 8795 448.56 5.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 457 2654 8622 496 2766 8836 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 457 2654 8622 496 2766 8836 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 457 2654 8622 496 2766 8836 Mean (pg/ml) 457 2654 8622 496 2766 8836 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 Standard deviation 20.57 145.97 439.72 19.34 127.24 494.82 CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6% CV (%) 4.5% 5.5% 5.1% 3.9% 4.6% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094 O.D. 0.052 0.123 0.255 0.462 0.775 1.343 1.769 2.094

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.