Human ACP6 ELISA Kit PicoKine®

Human ACP6 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ACP6 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Lysophosphatidic acid phosphatase type 6 is an acid phosphatase enzyme that is encoded in humans by the ACP6 gene. This gene encodes a member of the histidine acid phosphatase protein family. The encoded protein hydrolyzes lysophosphatidic acid, which is involved in G protein-coupled receptor signaling, lipid raft modulation, and in balancing lipid composition within the cell. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 28 29 24 27 0.95 3.6% Sample 2 166 157 169 174 167 4.83 2.9% Sample 3 457 479 485 460 470 14.58 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 28 29 24 27 0.95 3.6% Sample 1 25 28 29 24 27 0.95 3.6% Sample 2 166 157 169 174 167 4.83 2.9% Sample 2 166 157 169 174 167 4.83 2.9% Sample 3 457 479 485 460 470 14.58 3.1% Sample 3 457 479 485 460 470 14.58 3.1%

Gene Name

ACP6

Gene ID

51205

UniProt

Q9NPH0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: E33-E428

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 166 457 29 163 414 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 166 457 29 163 414 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 25 166 457 29 163 414 Mean (pg/ml) 25 166 457 29 163 414 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 Standard deviation 1.4 9.46 24.22 1.04 7.99 18.63 CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5% CV (%) 5.6% 5.7% 5.3% 3.6% 4.9% 4.5%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ACP6

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Acid phosphatase 6, lysophosphatidic

Protein Name

Lysophosphatidic acid phosphatase type 6

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321 O.D. 0.059 0.138 0.212 0.332 0.56 0.969 1.617 2.321

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.