Human ACP5 ELISA Kit PicoKine®

Tartrate-resistant acid phosphatase (TRAP or TRAPase), also called acid phosphatase 5, tartrate resistant (ACP5), is a glycosylated monomeric metalloprotein enzyme expressed in mammals. This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L (+) -tartrate.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 92 94 98 92 3.5 3.8% Sample 2 352 342 331 295 330 13.86 4.2% Sample 3 865 875 795 791 832 45.73 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 92 94 98 92 3.5 3.8% Sample 1 84 92 94 98 92 3.5 3.8% Sample 2 352 342 331 295 330 13.86 4.2% Sample 2 352 342 331 295 330 13.86 4.2% Sample 3 865 875 795 791 832 45.73 5.5% Sample 3 865 875 795 791 832 45.73 5.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 352 865 78 296 843 Standard deviation 4.54 20.77 36.33 3.35 17.17 52.27 CV (%) 5.4% 5.9% 4.2% 4.3% 5.8% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 352 865 78 296 843 Standard deviation 4.54 20.77 36.33 3.35 17.17 52.27 CV (%) 5.4% 5.9% 4.2% 4.3% 5.8% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 352 865 78 296 843 Mean (pg/ml) 84 352 865 78 296 843 Standard deviation 4.54 20.77 36.33 3.35 17.17 52.27 Standard deviation 4.54 20.77 36.33 3.35 17.17 52.27 CV (%) 5.4% 5.9% 4.2% 4.3% 5.8% 6.2% CV (%) 5.4% 5.9% 4.2% 4.3% 5.8% 6.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046 O.D. 0.015 0.062 0.099 0.185 0.329 0.625 1.179 2.046

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.