Human ZAG/AZGP1 ELISA Kit PicoKine®

Zinc-alpha-2-glycoprotein is a protein that in humans is encoded by the AZGP1 gene. Alpha-2-glycoprotein, also known as AZGP1, belongs to the MHC class I family. It can be detected in body fluids such as serum, sweat, and seminal and breast cyst fluids. It has been shown that alpha-2-glycoprotein can stimulate lipolysis by adipocytes in vivo and in vitro. Thus it is believed that alpha-2-glycoprotein plays an important role in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and it also regulates melanin production by normal and malignant melanocytes. Alpha-2-glycoprotein is produced by both white and brown fat adipocytes and may act in a local autocrine fashion in the reduction of adiposity in cachexia.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 452 501 486 477 479 21.56 4.5% Sample 2 2653 2854 2963 2832 2826 135.62 4.8% Sample 3 8848 8512 9041 8614 8754 533.98 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 452 501 486 477 479 21.56 4.5% Sample 1 452 501 486 477 479 21.56 4.5% Sample 2 2653 2854 2963 2832 2826 135.62 4.8% Sample 2 2653 2854 2963 2832 2826 135.62 4.8% Sample 3 8848 8512 9041 8614 8754 533.98 6.1% Sample 3 8848 8512 9041 8614 8754 533.98 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 452 2653 8848 445 2841 9012 Standard deviation 24.86 124.69 637.06 32.93 159.1 720.93 CV (%) 5.5% 4.7% 7.2% 7.4% 5.6% 8.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 452 2653 8848 445 2841 9012 Standard deviation 24.86 124.69 637.06 32.93 159.1 720.93 CV (%) 5.5% 4.7% 7.2% 7.4% 5.6% 8.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 452 2653 8848 445 2841 9012 Mean (pg/ml) 452 2653 8848 445 2841 9012 Standard deviation 24.86 124.69 637.06 32.93 159.1 720.93 Standard deviation 24.86 124.69 637.06 32.93 159.1 720.93 CV (%) 5.5% 4.7% 7.2% 7.4% 5.6% 8.0% CV (%) 5.5% 4.7% 7.2% 7.4% 5.6% 8.0%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.076 0.119 0.21 0.384 0.666 1.22 1.977 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.076 0.119 0.21 0.384 0.666 1.22 1.977 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.076 0.119 0.21 0.384 0.666 1.22 1.977 O.D. 0.035 0.076 0.119 0.21 0.384 0.666 1.22 1.977

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.